Transcription
- Initiation
RNA Polymerase binds to the 'promoter region'. In eukaryotic DNA, it is the sequence TATAAA. It is located 25 nucleotides upstream of the transcription site.
- Elongation
RNA Polymerase causes the two DNA strands to separate. It then adds nucleotides to the nucleotides of the template strand to make the RNA strand.
5' to 3' direction.
the 5' end is capped at the beginning of transcription.
- Termination
Poly-A tail ('AAAA') is attached to 3' end of mRNA.
Transcription creates a pre-mRNA strand.
Introns are longer sequences of nucleotides that do not code for anything. (Like "anything we know about yet"?) They are removed by RNA splicing.
Exons are shorter sequences of nucleotides code for the protein that will be made.
The resulting RNA is mRNA (messenger RNA)
Translation